Please use this identifier to cite or link to this item:
|Title:||Bilberry Adulteration Using the Food Dye Amaranth||Contributor(s):||Penman, K (author); Halstead, C (author); Matthias, A (author); De Voss, J (author); Stuthe, J (author); Bone, Kerry (author); Lehmann, R (author)||Publication Date:||2006||DOI:||10.1021/jf061387d||Handle Link:||https://hdl.handle.net/1959.11/1398||Abstract:||'Vaccinium myrtillus' or bilberry fruit is a commonly used herbal product. The usual method of determining the anthocyanin content is a single-wavelength spectrophotometric assay. Using this method, anthocyanin levels of two extracts were found to be 25% as claimed by the manufacturers. When high-performance liquid chromatography (HPLC) was used, however, one extract was found to contain 9% anthocyanins probably not derived from 'V. myrtillus' but from an adulterant. This adulterant was subsequently identified, using HPLC, mass spectroscopy, and nuclear magnetic resonance, as amaranth, that is, 3-hydroxy-4-[(4-sulfo-1-naphthalenyl)azo]-2,7-naphthalenedisulfonic acid trisodium saltsa synthetic dark red sulfonic acid based naphthylazo dye. As described in this study, if deliberate adulteration occurs in an extract, a single-wavelength spectrophotometric assay is inadequate to accurately determine the levels of compounds such as anthocyanins. Detection of deliberate adulteration in commercial samples thus requires the use of alternative, more sophisticated, methods of analysis such as HPLC with photodiode array detection as a minimum.||Publication Type:||Journal Article||Source of Publication:||Journal of Agricultural and Food Chemistry, v.54, p. 7378-7382||Publisher:||American Chemical Society||Place of Publication:||Washington, United States of America||ISSN:||0021-8561||Field of Research (FOR):||220107 Professional Ethics (incl police and research ethics)||Peer Reviewed:||Yes||HERDC Category Description:||C1 Refereed Article in a Scholarly Journal||Statistics to Oct 2018:||Visitors: 382
|Appears in Collections:||Journal Article|
Files in This Item:
checked on Nov 26, 2018
checked on Mar 4, 2019
Items in Research UNE are protected by copyright, with all rights reserved, unless otherwise indicated.